Phytochemical Analysis and Biological Evaluation of Hypericum linarioides Bosse: in Vitro and in Silico Studies

Altay A., Yeniçeri E., Taslimi P., Taşkın Tok T., Yılmaz M. A., Köksal E.

CHEMISTRYSELECT, cilt.7, sa.3, ss.1-10, 2022 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 7 Sayı: 3
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1002/slct.202200039
  • Dergi Adı: CHEMISTRYSELECT
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier
  • Sayfa Sayıları: ss.1-10
  • Erzincan Binali Yıldırım Üniversitesi Adresli: Evet

Özet

The aim of this studyis to determinethe phenoliccompositionofHypericumlinarioidesBosse,to assessits variousbiologicalproperties,and finallyto confirmthe experimentaldataofenzymeinhibitionsvia dockingstudies.LC-MS/MSanalysisperformedover methanolextract(ME) revealedthe presenceof27 phytochemicals.AntioxidantactivityresultsindicatedthatME had the strongestDPPHradicalscavengingand ferricionreducingcapacitythanwaterand hexaneextracts.XTT assaysrevealedthe strongestanti-proliferativeactivityof ME againstMCF-7and HT-29cancercells.Flowcytometryresultsalsoconfirmedthe notableapoptoticeffectof ME on MCF-7cellsthroughmitochondria-dependentpathway.Enzymeactivityresultsexhibitedthe mostpromisingacetylcholinesteraseandα-glycosidaseinhibitoryactivitiesof the ME. Moleculardockingcalculationsofα-glycosidasewith the majorcompounds(1, 15,17 and 19) indicatedthat17 (isoquercitrin)had the bestdockingbindingenergy(10.92kcal/mol)withappropriatebindinginteractions.