The effects of melatonin in glutamate-induced neurotoxicity of rat cerebellar granular cell culture

Gepdiremen A., Düzenli S., HACIMÜFTÜOĞLU A., Bulucu D., Süleyman H.

Japanese Journal of Pharmacology, cilt.84, sa.4, ss.467-469, 2000 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 84 Sayı: 4
  • Basım Tarihi: 2000
  • Doi Numarası: 10.1254/jjp.84.467
  • Dergi Adı: Japanese Journal of Pharmacology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.467-469
  • Anahtar Kelimeler: Glutamate, Melatonin, Neuroprotection
  • Erzincan Binali Yıldırım Üniversitesi Adresli: Hayır

Özet

In the present study, melatonin was tested in subsequent doses in glutamate induced neurotoxicity in cerebellar granular cell culture of rat pups. Glutamate at 10-7 M was found to induce neuronal cell death. The dead cell score was 2.75 ± 0.7 in the control, while it was found to be 35.12 ± 1.8 in the glutamate-administered group (P<0.0001). Melatonin very potently blocked glutamate neurotoxicity at all doses tested, with 10-3 M, the highest dose tested, being the most effective. Glutamate may exert a neuroprotective effect by blocking one or more steps of the oxidation cascade in neurons and this effect may be blocked by melatonin.