Isolation, characterization and anticancer activity of secondary metabolites from Verbascum speciosum

Demirci S., ALP C., AKŞİT H., Ulutaş Y., ALTAY A., Yeniçeri E., ...Daha Fazla

Chemical Biology and Drug Design, cilt.101, sa.6, ss.1273-1282, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 101 Sayı: 6
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1111/cbdd.14211
  • Dergi Adı: Chemical Biology and Drug Design
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1273-1282
  • Anahtar Kelimeler: ajugol, anticancer activity, aucubin, iridoid glucosides, natural products, phenylethanoids glucosides, poliumoside, preparative HPLC, Scrophulariaceae, verbascoside (= acteoside), Verbascum speciosum
  • Erzincan Binali Yıldırım Üniversitesi Adresli: Evet

Özet

Herein, two iridoid glucosides aucubin (1) and ajugol (2), and two phenyl ethanoids, verbascoside (3) and poliumoside (4) were isolated from the methanol extract of the aerial parts of Verbascum speciosum and used to study about their anticancer activity for the first time. The structures of all compounds were elucidated using spectroscopic data (IR, 1D and 2D NMR, LC-TOF/MS). Antiproliferative activities of Aucubun (1) and Verbascoside (3) were tested against A-549 (human colon cancer), MDA-MD-453 (human breast cancer) and 3T3-L1 (mouse fibroblast)cell lines by XTT assay. In addition, the anticarcer mechanism of action of aucubin (1) was investigated on MDA-MB-453 cells for the first time. XTT result showed that both applied compounds exhibited antiproliferative effect at different dose ranges depending on the cancer type, as well as selectivity between cancer and healty cell lines. Flow cytometry analyzes revealed that aucubin (1) exerts its cytotoxic effect in MDA-MB-453 cells by directing cells to early apoptosis and inhibiting the P13K/AKT signaling pathway.