Akademik Veri Yönetim Sistemi
3rd International Eurasian Conference on Science, Engineering and Technology (EurasianSciEnTech 2021), Ankara, Türkiye, 15 - 17 Aralık 2021, ss.717-724
In this study, antioxidant activity and antiproliferative effects of various extracts (hexane, water and methanol)
from the aerial parts of Hypericum scabrum L. (H. scabrum) were investigated. Total phenolic content of the
extracts was determined by Folin Ciocalteu assay. Radical scavenging and metal reducing capacity of the
extracts were determined by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power
(FRAP) assays. The antiproliferative power of the extracts were evaluated on human colon cancer (HT-29)
and healthy continuous (GF-1) cell lines via 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-
Carboxanilide (XTT) assay. Folin Ciocalteu results showed that the methanol extract (ME) was the richest in
phenolic content (13.20 mg GAE/g extract), followed by water extract (WE) and hexane extract (HE). ME also exhibited much higher DPPH radical scavenging and iron reducing capacity than WE and HE. XTT results indicated that we and ME and WE showed dose-dependent
antiproliferative effects between 100 and 250 on HT-29 cells, while they did not exhibit any
cytotoxicity on GF-1 cells up to 500 . In conclusion, all these preliminary results assume that H. scabrum
creates the impression that it can be considered as a potential source in the treatment of colon cancer with its
rich phenolic content, moderate antioxidant capacity, and remarkable antiproliferative power.
Keywords: Hypericum scabrum L., Phenolic content, Antioxidant activity, Cytotoxicity